iscripttm advanced cdna synthesis kit buffer Search Results


g dna buffer  (Bio-Rad)
99
Bio-Rad g dna buffer
G Dna Buffer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/g dna buffer/product/Bio-Rad
Average 99 stars, based on 1 article reviews
g dna buffer - by Bioz Stars, 2026-03
99/100 stars
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iscripttm advanced cdna synthesis kit  (Bio-Rad)
99
Bio-Rad iscripttm advanced cdna synthesis kit
Iscripttm Advanced Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iscripttm advanced cdna synthesis kit/product/Bio-Rad
Average 99 stars, based on 1 article reviews
iscripttm advanced cdna synthesis kit - by Bioz Stars, 2026-03
99/100 stars
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rneasy mini kit  (Qiagen)
90
Qiagen rneasy mini kit
Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rneasy mini kit/product/Qiagen
Average 90 stars, based on 1 article reviews
rneasy mini kit - by Bioz Stars, 2026-03
90/100 stars
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rna lysis buffer rneasy plus mini kit  (Qiagen)
90
Qiagen rna lysis buffer rneasy plus mini kit
Rna Lysis Buffer Rneasy Plus Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rna lysis buffer rneasy plus mini kit/product/Qiagen
Average 90 stars, based on 1 article reviews
rna lysis buffer rneasy plus mini kit - by Bioz Stars, 2026-03
90/100 stars
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rlt buffer qiagen  (Qiagen)
90
Qiagen rlt buffer qiagen
Rlt Buffer Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rlt buffer qiagen/product/Qiagen
Average 90 stars, based on 1 article reviews
rlt buffer qiagen - by Bioz Stars, 2026-03
90/100 stars
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mirneasy serum/plasma advanced kit  (Qiagen)
90
Qiagen mirneasy serum/plasma advanced kit
Mirneasy Serum/Plasma Advanced Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mirneasy serum/plasma advanced kit/product/Qiagen
Average 90 stars, based on 1 article reviews
mirneasy serum/plasma advanced kit - by Bioz Stars, 2026-03
90/100 stars
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fastlane cell cdna kit  (Qiagen)
90
Qiagen fastlane cell cdna kit
M14 cell line (10 4 cells) was cultured and lysed with the Trizol reagent, in-house and Bio-Rad cell-to-Ct reagents. After reverse transcription, the <t>cDNA</t> samples were amplified by RPS8, ZNF706, RNF167, hsa-miR-29a, hsa-miR-197, hsa-miR-297 PCR assays. Graph bars represent (A) CVs and (B) detected Ct values of the biological quadruplicates. The error bars referred to the S.E.M and the significant differences in CVs between the Trizol reagent and cell-to-Ct reagents were calculated using unpaired, two tailed student's t-test. *, p<0.05; **, p<0.005.
Fastlane Cell Cdna Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fastlane cell cdna kit/product/Qiagen
Average 90 stars, based on 1 article reviews
fastlane cell cdna kit - by Bioz Stars, 2026-03
90/100 stars
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buffer of cell lysate for reverse transcription  (Signosis Inc)
90
Signosis Inc buffer of cell lysate for reverse transcription
M14 cell line (10 4 cells) was cultured and lysed with the Trizol reagent, in-house and Bio-Rad cell-to-Ct reagents. After reverse transcription, the <t>cDNA</t> samples were amplified by RPS8, ZNF706, RNF167, hsa-miR-29a, hsa-miR-197, hsa-miR-297 PCR assays. Graph bars represent (A) CVs and (B) detected Ct values of the biological quadruplicates. The error bars referred to the S.E.M and the significant differences in CVs between the Trizol reagent and cell-to-Ct reagents were calculated using unpaired, two tailed student's t-test. *, p<0.05; **, p<0.005.
Buffer Of Cell Lysate For Reverse Transcription, supplied by Signosis Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer of cell lysate for reverse transcription/product/Signosis Inc
Average 90 stars, based on 1 article reviews
buffer of cell lysate for reverse transcription - by Bioz Stars, 2026-03
90/100 stars
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buffer rlt  (Qiagen)
96
Qiagen buffer rlt
M14 cell line (10 4 cells) was cultured and lysed with the Trizol reagent, in-house and Bio-Rad cell-to-Ct reagents. After reverse transcription, the <t>cDNA</t> samples were amplified by RPS8, ZNF706, RNF167, hsa-miR-29a, hsa-miR-197, hsa-miR-297 PCR assays. Graph bars represent (A) CVs and (B) detected Ct values of the biological quadruplicates. The error bars referred to the S.E.M and the significant differences in CVs between the Trizol reagent and cell-to-Ct reagents were calculated using unpaired, two tailed student's t-test. *, p<0.05; **, p<0.005.
Buffer Rlt, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/buffer rlt/product/Qiagen
Average 96 stars, based on 1 article reviews
buffer rlt - by Bioz Stars, 2026-03
96/100 stars
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total rna isolation kit  (GeneDireX Inc)
90
GeneDireX Inc total rna isolation kit
M14 cell line (10 4 cells) was cultured and lysed with the Trizol reagent, in-house and Bio-Rad cell-to-Ct reagents. After reverse transcription, the <t>cDNA</t> samples were amplified by RPS8, ZNF706, RNF167, hsa-miR-29a, hsa-miR-197, hsa-miR-297 PCR assays. Graph bars represent (A) CVs and (B) detected Ct values of the biological quadruplicates. The error bars referred to the S.E.M and the significant differences in CVs between the Trizol reagent and cell-to-Ct reagents were calculated using unpaired, two tailed student's t-test. *, p<0.05; **, p<0.005.
Total Rna Isolation Kit, supplied by GeneDireX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total rna isolation kit/product/GeneDireX Inc
Average 90 stars, based on 1 article reviews
total rna isolation kit - by Bioz Stars, 2026-03
90/100 stars
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lysis buffer  (Qiagen)
90
Qiagen lysis buffer
M14 cell line (10 4 cells) was cultured and lysed with the Trizol reagent, in-house and Bio-Rad cell-to-Ct reagents. After reverse transcription, the <t>cDNA</t> samples were amplified by RPS8, ZNF706, RNF167, hsa-miR-29a, hsa-miR-197, hsa-miR-297 PCR assays. Graph bars represent (A) CVs and (B) detected Ct values of the biological quadruplicates. The error bars referred to the S.E.M and the significant differences in CVs between the Trizol reagent and cell-to-Ct reagents were calculated using unpaired, two tailed student's t-test. *, p<0.05; **, p<0.005.
Lysis Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer/product/Qiagen
Average 90 stars, based on 1 article reviews
lysis buffer - by Bioz Stars, 2026-03
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Image Search Results


M14 cell line (10 4 cells) was cultured and lysed with the Trizol reagent, in-house and Bio-Rad cell-to-Ct reagents. After reverse transcription, the cDNA samples were amplified by RPS8, ZNF706, RNF167, hsa-miR-29a, hsa-miR-197, hsa-miR-297 PCR assays. Graph bars represent (A) CVs and (B) detected Ct values of the biological quadruplicates. The error bars referred to the S.E.M and the significant differences in CVs between the Trizol reagent and cell-to-Ct reagents were calculated using unpaired, two tailed student's t-test. *, p<0.05; **, p<0.005.

Journal: PLoS ONE

Article Title: Direct Quantification of mRNA and miRNA from Cell Lysates Using Reverse Transcription Real Time PCR: A Multidimensional Analysis of the Performance of Reagents and Workflows

doi: 10.1371/journal.pone.0072463

Figure Lengend Snippet: M14 cell line (10 4 cells) was cultured and lysed with the Trizol reagent, in-house and Bio-Rad cell-to-Ct reagents. After reverse transcription, the cDNA samples were amplified by RPS8, ZNF706, RNF167, hsa-miR-29a, hsa-miR-197, hsa-miR-297 PCR assays. Graph bars represent (A) CVs and (B) detected Ct values of the biological quadruplicates. The error bars referred to the S.E.M and the significant differences in CVs between the Trizol reagent and cell-to-Ct reagents were calculated using unpaired, two tailed student's t-test. *, p<0.05; **, p<0.005.

Article Snippet: The 7 cell lines in a 96-well plate (2×10 4 cells per well, biological quadruplicates) were lysed with RealTime Ready Cell Lysis Kit (Roche), FastLane Cell cDNA Kit (Qiagen), Buffer of Cell Lysate for Reverse Transcription (Signosis BioSignal Capture), iScriptTM RT-qPCR Sample Preparation Reagent (Bio-Rad Laboratories), or MicroRNA Cells-to-CTTM Kit (ABI Ambion) according to manufacturer recommendation.

Techniques: Cell Culture, Reverse Transcription, Amplification, Two Tailed Test

M14 cell line cultured in 96-wells at various densities (10, 100, 1000, 10,000 and 100,000 cells per well) were lysed directly by (A) in-house or (B) Bio-Rad lysis buffer. The cDNA samples generated from cell lysates were amplified by PCR assays from set 2. Standard curves for isolated RNAs were plotted as Ct versus Log (cells per RT). The experiments were conducted with biological duplicates.

Journal: PLoS ONE

Article Title: Direct Quantification of mRNA and miRNA from Cell Lysates Using Reverse Transcription Real Time PCR: A Multidimensional Analysis of the Performance of Reagents and Workflows

doi: 10.1371/journal.pone.0072463

Figure Lengend Snippet: M14 cell line cultured in 96-wells at various densities (10, 100, 1000, 10,000 and 100,000 cells per well) were lysed directly by (A) in-house or (B) Bio-Rad lysis buffer. The cDNA samples generated from cell lysates were amplified by PCR assays from set 2. Standard curves for isolated RNAs were plotted as Ct versus Log (cells per RT). The experiments were conducted with biological duplicates.

Article Snippet: The 7 cell lines in a 96-well plate (2×10 4 cells per well, biological quadruplicates) were lysed with RealTime Ready Cell Lysis Kit (Roche), FastLane Cell cDNA Kit (Qiagen), Buffer of Cell Lysate for Reverse Transcription (Signosis BioSignal Capture), iScriptTM RT-qPCR Sample Preparation Reagent (Bio-Rad Laboratories), or MicroRNA Cells-to-CTTM Kit (ABI Ambion) according to manufacturer recommendation.

Techniques: Cell Culture, Lysis, Generated, Amplification, Isolation

M14 cultured in 96-wells at 10 4 cells per well (biological triplicates) were lysed directly by (A) in-house or (B) Bio-Rad lysis buffers and incubated at room temperature across various durations (5, 10, 30, 60, 120, 240 min). The cDNA samples generated from cell lysates were amplified by PCR assays from set 2. Ct was plotted against duration of incubation to examine the stability of RNA in room temperature. The experiments were conducted with biological duplicates.

Journal: PLoS ONE

Article Title: Direct Quantification of mRNA and miRNA from Cell Lysates Using Reverse Transcription Real Time PCR: A Multidimensional Analysis of the Performance of Reagents and Workflows

doi: 10.1371/journal.pone.0072463

Figure Lengend Snippet: M14 cultured in 96-wells at 10 4 cells per well (biological triplicates) were lysed directly by (A) in-house or (B) Bio-Rad lysis buffers and incubated at room temperature across various durations (5, 10, 30, 60, 120, 240 min). The cDNA samples generated from cell lysates were amplified by PCR assays from set 2. Ct was plotted against duration of incubation to examine the stability of RNA in room temperature. The experiments were conducted with biological duplicates.

Article Snippet: The 7 cell lines in a 96-well plate (2×10 4 cells per well, biological quadruplicates) were lysed with RealTime Ready Cell Lysis Kit (Roche), FastLane Cell cDNA Kit (Qiagen), Buffer of Cell Lysate for Reverse Transcription (Signosis BioSignal Capture), iScriptTM RT-qPCR Sample Preparation Reagent (Bio-Rad Laboratories), or MicroRNA Cells-to-CTTM Kit (ABI Ambion) according to manufacturer recommendation.

Techniques: Cell Culture, Lysis, Incubation, Generated, Amplification